Wolfe Laboratories - From Concept to Creation with Confidence

In-vitro ADME Screens
   Aqueous Solubility
   Plasma Stability
   Protein Binding
   Microsome Stability
   CYP450
   Permeability
Pharmacokinetic Analysis
  Bioanalytical Method Development
  Biological Fluid Sample Analysis
  Tissue Analysis
Metabolite Identification

>	CASE STUDY 1 Characterization of a Protein Therapeutic Using Mass Spectrometry
>	CASE STUDY 2 Discovery Pharmacokinetic (PK) Studies to iIdentify a Lead Compound
>	CASE STUDY 3 Characterizing Impurities in a New Formulation or Device

Plasma Stability

The stability of test compounds in plasma is an important parameter, which not only affects in vivo results, but also the bioanalytical assay strategy and design. Investigation of plasma stability should be performed early in the discovery process in order to assess potential degradation and/or protein binding issues.

A solution of test compound in plasma is prepared and incubated for a predetermined time period. Aliquots are removed at pre-defined time points and analyzed by LC/MS/MS. The peak area for the parent compound is compared to the time zero sample in order to assess the amount of compound still available.